ABSTRACT
Objective To investigate the effect of syntenin-1 on the assembly of hepatitis C virus (HCV) particles. Methods The content of syntenin-1 in human primary hepatocytes was detected by Western blotting analysis. Human hepatocellular carcinoma cell line Huh7.5.1 was transfected with lentiviral plasmids to construct syntenin-1 overexpressed cell line and green fluorescent protein (GFP) overexpressed cell line. After transferring HCV RNA into Huh7.5.1 cells, syntenin-1 overexpressed cells and GFP overexpressed cells by electroporation, the effects of syntenin-1 overexpression on HCV in the cells were investigated in terms of RNA replication, viral protein contents and secretion of infectious virus particles by luciferase analysis, Western blotting and virus titer, respectively. The density of viral particles was assessed by isopycnic ultracentrifugation to analyze the distributions of HCV RNA and infective titer. Results The contents of syntenin-1 in three human primary hepatocytes were higher than that in the Huh7.5.1 cells (P0.01). Syntenin-1 overexpression had no effect on HCV RNA replication in host cells; the infectivity of HCV derived from syntenin-1 overexpressed cells was not significantly different compared with the Huh7.5.1 cells or GFP overexpressed cells. In syntenin-1 overexpressed cell culture supernatants, some infectious HCV particles mainly concentrated in the region of concentration of 1.08-1.16 g/mL gradually transferred to the low-density region of 1.01-1.02 g/mL. Conclusion Syntenin-1 overexpression alters the distribution of density components of infectious HCV particles.
ABSTRACT
The growing interest of the pharmaceutical industry in Near Infrared-Chemical Imaging (NIR-CI) is a result of its high usefulness for quality control analyses of drugs throughout their production process (particularly of its non-destructive nature and expeditious data acquisition).In this work,the concentration and distribution of the major and minor components of pharmaceutical tablets are determined and the spatial distribution from the internal and external sides has been obtained.In addition,the same NIR-CI allowed the coating thickness and its surface distribution to be quantified.Images were processed to extract the target data and calibration models constructed using the Partial Least Squares (PLS) algorithms.The concentrations of Active Pharmaceutical Ingredient (API) and excipients obtained for uncoated cores were essentially identical to the nominal values of the pharmaceutical formulation.But the predictive ability of the calibration models applied to the coated tablets decreased as the coating thickness increased.
ABSTRACT
BACKGROUND: Because the platelet parameters have recently been used as indicators for various clinical conditions, there is a need to accurately estimate the platelet parameters by using an automated hematology analyzer. The aim of this study was to investigate an effect of a platelet transfusion on such platelet parameters as the mean platelet volume (MPV), the platelet volume distribution width (PDW), the mean platelet component (MPC) and the platelet component distribution width (PCDW) in transfused patients. METHODS: The study subjects were 25 patients who were admitted to the Department of Hematology & Oncology and they had been transfused with platelets. CD62P labeling was performed in the remaining portions of single donor platelets products (SDP) and the pooled platelet concentrates (PC) just before the SDP and PC were released. The platelet parameters were determined using the ADVIA 120 and the whole blood samples from the patients before the platelet transfusions and within 10~16 hours after the transfusion. RESULTS: There were no significant difference of all the platelet parameters between the SDP (n=21) and the pooled PC (n=8). The MPC and PCDW of the SDP and the PC were significantly lower than those of the samples from the patients before transfusion. However, the PCDW of the samples from the patients after transfusion was significantly lower than that before transfusion. CONCLUSION: Because platelet transfusions lower the value of the PCDW in patients, the laboratory staff and clinicians should be aware of this when interpreting the PCDW.